Recombinant Human Taste receptor type 1 member 2 (TAS1R2), Truncated

Amino acids 20-566 constitute the expression domain of recombinant Human TAS1R2. This TAS1R2 protein is theoretically predicted to have a molecular weight of 67.6 kDa. Expression of this TAS1R2 protein is conducted in e.coli. The TAS1R2 coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant TAS1R2 protein in following stages of expression and purification.The human taste receptor type 1 member 2 (TAS1R2) is a G protein-coupled receptor (GPCR) that is integral to the detection of sweet taste. TAS1R2, in conjunction with TAS1R3, forms a heterodimeric receptor complex known as T1R2/T1R3, responsible for recognizing various sweet compounds, including sugars and artificial sweeteners. Activation of TAS1R2/TAS1R3 initiates intracellular signaling pathways, resulting in the perception of sweetness. TAS1R2 is predominantly expressed in taste receptor cells on the tongue, contributing to the pleasurable experience of sweet flavors in food. Understanding the function of TAS1R2 is essential for comprehending the molecular mechanisms underlying sweet taste perception and has implications for the development of sweeteners and flavor enhancers.