Recombinant Human Ubiquitin carboxyl-terminal hydrolase 5 (USP5), Truncated

Uniprot Id

P45974

Target Species

Human

Target Name

USP5

Target Full Name

Ubiquitin carboxyl-terminal hydrolase 5

Target Function

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Target Protein Families

Peptidase C19 family

Target Synonyms

Deubiquitinating enzyme 5; Isopeptidase T; ISOT 1; ISOT; Ubiquitin carboxyl-terminal hydrolase 5; Ubiquitin isopeptidase T; Ubiquitin specific peptidase 5 (isopeptidase T); Ubiquitin specific peptidase 5; Ubiquitin specific processing protease 5; Ubiquitin specific protease 5 (isopeptidase T); Ubiquitin specific protease 5 (ubiquitin isopeptidase T); Ubiquitin thioesterase 5; Ubiquitin thiolesterase 5; Ubiquitin-specific-processing protease 5; UBP5_HUMAN; Usp5

Target Background

Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).