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In expressing the recombinant Human USP6 protein, e.coli cells are transfected with a DNA expression vector that harbors the gene for the desired protein (348-535aa). The cultured cells then express the intended protein. The protein is equipped with a N-terminal 6xHis-KSI tag. The recombinant Human USP6 protein is collected and purified from the cell lysate through affinity purification, achieving a purity surpassing 85%, as confirmed by SDS-PAGE.
In expressing the recombinant Human USP6 protein, e.coli cells are transfected with a DNA expression vector that harbors the gene for the desired protein (348-535aa). The cultured cells then express the intended protein. The protein is equipped with a N-terminal 6xHis-KSI tag. The recombinant Human USP6 protein is collected and purified from the cell lysate through affinity purification, achieving a purity surpassing 85%, as confirmed by SDS-PAGE.
| Cat.No | ACP01652 | Target Name | USP6 |
|---|---|---|---|
| Target Synonyms | (Deubiquitinating enzyme 6)(Proto-oncogene TRE-2)(Ubiquitin thioesterase 6)(Ubiquitin-specific-processing protease 6) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 348-535aa |
| Mol Weight | 36.0 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P35125 |
|---|
Uniprot Id
P35125
Target Species
Human
Target Name
USP6
Target Full Name
Ubiquitin carboxyl-terminal hydrolase 6
Target Function
Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
Target Involvement
A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation (PubMed:15026324).
Target Subcellular Location
Cell membrane. Cytoplasm. Endosome. Note=Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.
Target Protein Families
Peptidase C19 family
Target Tissue Specificity
Testis specific. Expressed in various cancer cell lines.
Target Research Area
Cell Biology
Target Synonyms
Deubiquitinating enzyme 6; HRP1; Proto-oncogene TRE-2; TRE17; TRE2; Ubiquitin carboxyl-terminal hydrolase 6; Ubiquitin specific protease 6; Ubiquitin thiolesterase 6; Ubiquitin-specific-processing protease 6; UBP6_HUMAN; USP6
Target Background
Enables thiol-dependent deubiquitinase. Involved in protein deubiquitination and regulation of vesicle-mediated transport. Located in plasma membrane and recycling endosome.
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