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The recombinant Human UAP1 protein production in Yeast cells involves the integration of a DNA fragment encoding the Human UAP1 protein (1-522aa) into a plasmid vector, which is then introduced into Yeast cells. Following screening, positive cells are cultured and induced to synthesize the UAP1 protein. A N-terminal 6xHis tag is attached to the protein. Cell lysis is carried out to harvest the recombinant Human UAP1 protein, which is purified through affinity purification and then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human UAP1 protein reaches over 90%.
The recombinant Human UAP1 protein production in Yeast cells involves the integration of a DNA fragment encoding the Human UAP1 protein (1-522aa) into a plasmid vector, which is then introduced into Yeast cells. Following screening, positive cells are cultured and induced to synthesize the UAP1 protein. A N-terminal 6xHis tag is attached to the protein. Cell lysis is carried out to harvest the recombinant Human UAP1 protein, which is purified through affinity purification and then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human UAP1 protein reaches over 90%.
| Cat.No | ACP02541 | Target Name | UAP1 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Yeast |
| Expression Range | 1-522aa | Mol Weight | 60.8kDa |
| Protein Length | Full length | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q16222 |
|---|
Uniprot Id
Q16222
Target Species
Human
Target Name
UAP1
Target Full Name
UDP-N-acetylhexosamine pyrophosphorylase
Target Function
Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.
Target Subcellular Location
Cytoplasm. Note=In spermatozoa, localized to the principal piece of the tail, the neck region of the head and to a lesser extent, the midpiece of the tail.
Target Protein Families
UDPGP type 1 family
Target Tissue Specificity
Widely expressed. Isoform AGX1 is more abundant in testis than isoform AGX2, while isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. Expressed at low level in placenta, muscle and liver.
Target Research Area
Signal Transduction
Target Synonyms
AGX 1; AGX; AGX-1; AGX-2; AGX1; Antigen X; AntigenX; SPAG 2; SPAG2; Sperm associated antigen 2; Sperm-associated antigen 2; UAP 1; uap1; UAP1_HUMAN; UDP N acetylhexosamine pyrophosphorylase; UDP N acteylglucosamine pyrophosphorylase 1; UDP-N-acetylglucosamine pyrophosphorylase
Target Background
Enables identical protein binding activity. Predicted to be involved in UDP-N-acetylglucosamine biosynthetic process. Located in cytosol; nucleoplasm; and plasma membrane.
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