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The DNA fragment encoding the 20-764aa of the human KDR protein was fused with N-terminal GST tag gene and then was inserted into the expression vector, which was subsequently transformed into the E.coli for expression. The resulting product was further purified to obtain the recombinant human KDR protein. The purity of this recombinant KDR protein is greater than 90% assessed by Bandscan software analysis combined with SDS-PAGE. This KDR protein showed a band on the gel with a molecular weight of approximately 105 kDa.As a VEGF receptor, VEGFR-2 (also known as KDR, FLK1, CD309) is a type IV receptor tyrosine kinase. It plays major part in the regulation of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. In addition, KDR has been found closely related with the SHC2, Annexin A5, SHC1. The signaling involved in the KDR include Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. KDR exerts multiple biological effects through its interaction with other molecules. Of note, more researches suggested that KDR participates in the tumor growth and metastasis.
The DNA fragment encoding the 20-764aa of the human KDR protein was fused with N-terminal GST tag gene and then was inserted into the expression vector, which was subsequently transformed into the E.coli for expression. The resulting product was further purified to obtain the recombinant human KDR protein. The purity of this recombinant KDR protein is greater than 90% assessed by Bandscan software analysis combined with SDS-PAGE. This KDR protein showed a band on the gel with a molecular weight of approximately 105 kDa.As a VEGF receptor, VEGFR-2 (also known as KDR, FLK1, CD309) is a type IV receptor tyrosine kinase. It plays major part in the regulation of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. In addition, KDR has been found closely related with the SHC2, Annexin A5, SHC1. The signaling involved in the KDR include Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. KDR exerts multiple biological effects through its interaction with other molecules. Of note, more researches suggested that KDR participates in the tumor growth and metastasis.
| Cat.No | ACP04177 | Target Name | KDR |
|---|---|---|---|
| Target Synonyms | CD309; CD309 antigen; EC 2.7.10.1; Fetal liver kinase 1; FLK-1; FLK1; FLK1, mouse | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 20-764aa |
| Mol Weight | 110.3kDa | Protein Length | Extracellular Domain |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P35968 |
|---|
Uniprot Id
P35968
Target Species
Human
Target Name
KDR
Target Full Name
Vascular endothelial growth factor receptor 2
Target Function
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Target Involvement
Hemangioma, capillary infantile (HCI)
Target Subcellular Location
Cell junction. Endoplasmic reticulum. Cell membrane.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome. Note=Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.; [Isoform 2]: Secreted.; [Isoform 3]: Secreted.
Target Protein Families
Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
Target Tissue Specificity
Detected in cornea (at protein level). Widely expressed.
Target Research Area
Cancer
Target Synonyms
CD309; CD309 antigen; EC 2.7.10.1; Fetal liver kinase 1; FLK-1; FLK1; FLK1, mouse, homolog of; Kdr; Kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein tyrosine kinase receptor FLK1; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; Tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; VEGFR 2; VEGFR; VEGFR-2; VEGFR2; VGFR2_HUMAN
Target Background
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
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