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The production process of the recombinant human O-phosphoseryl-tRNA (Sec) selenium transferase (SEPSECS) involves the expression of a vector construct in yeast cells. To create this construct, the gene fragment encoding the full-length human SEPSECS protein (1-501aa) is cloned with a C-terminal 6xHis-tag into a plasmid vector. The resulting vector construct, harboring the desired gene, is then transfected into yeast cells. Transfected yeast cells are carefully selected using specific antibiotics and subsequently cultured under optimal conditions. The induction reagents aid in the expression of the recombinant human SEPSECS protein within the yeast cells. After expression, the yeast cells are harvested and lysed to release their cellular contents, including the recombinant SEPSECS protein. Purification of the recombinant SEPSECS protein is conducted from the cell lysate, yielding a purity level exceeding 85% as confirmed by SDS-PAGE analysis.
The production process of the recombinant human O-phosphoseryl-tRNA (Sec) selenium transferase (SEPSECS) involves the expression of a vector construct in yeast cells. To create this construct, the gene fragment encoding the full-length human SEPSECS protein (1-501aa) is cloned with a C-terminal 6xHis-tag into a plasmid vector. The resulting vector construct, harboring the desired gene, is then transfected into yeast cells. Transfected yeast cells are carefully selected using specific antibiotics and subsequently cultured under optimal conditions. The induction reagents aid in the expression of the recombinant human SEPSECS protein within the yeast cells. After expression, the yeast cells are harvested and lysed to release their cellular contents, including the recombinant SEPSECS protein. Purification of the recombinant SEPSECS protein is conducted from the cell lysate, yielding a purity level exceeding 85% as confirmed by SDS-PAGE analysis.
| Cat.No | ACP01133 | Target Name | SEPSECS |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Yeast |
| Expression Range | 1-501aa | Mol Weight | 56.5 kDa |
| Protein Length | Full length | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9HD40 |
|---|
Uniprot Id
Q9HD40
Target Species
Human
Target Name
SEPSECS
Target Full Name
O-phosphoseryl-tRNA(Sec) selenium transferase
Target Function
Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.
Target Involvement
Pontocerebellar hypoplasia 2D (PCH2D)
Target Subcellular Location
Cytoplasm.
Target Protein Families
SepSecS family
Target Tissue Specificity
Primarily expressed in liver, pancreas, kidney and lung. Overexpressed in PHA-stimulated T-cells.
Target Research Area
Epigenetics and Nuclear Signaling, Metabolism
Target Synonyms
DKFZp434B1417; Liver pancreas antigen; Liver-pancreas antigen; LP; MGC161491; O phosphoseryl tRNA(Sec) selenium transferase; O-phosphoseryl-tRNA(Sec) selenium transferase; Sec synthase; Selenocysteine synthase; Selenocysteinyl tRNA(Sec) synthase; Selenocysteinyl-tRNA(Sec) synthase; Sep (O phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase; Sep tRNA:Sec tRNA synthase; Sep-tRNA:Sec-tRNA synthase; SepSecS; SLA; SLA p35; SLA-p35; SLA/LP; SLA/LP autoantigen; Soluble liver antigen; Soluble liver antigen/liver pancreas antigen; Soluble Liver/Pancreas Antigen; SPCS_HUMAN; tRNA(Ser/Sec) associated antigenic protein; tRNA(Ser/Sec)-associated antigenic protein; TRNP48; UGA suppressor tRNA associated protein; UGA suppressor tRNA-associated protein
Target Background
The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).
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