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The recombinant Human ERCC3 protein production is achieved through the manipulation of desired gene expression in e.coli cells. The sequence (1-782aa) of foreign DNA is fused to an expression vector and then transformed into e.coli cells. The positive cells are selected and cultured to induce the expression of the desired protein. A N-terminal GST tag is fused to the protein. The recombinant Human ERCC3 protein is subjected to affinity purification. Its purity is over 85%, as measured by SDS-PAGE.
The recombinant Human ERCC3 protein production is achieved through the manipulation of desired gene expression in e.coli cells. The sequence (1-782aa) of foreign DNA is fused to an expression vector and then transformed into e.coli cells. The positive cells are selected and cultured to induce the expression of the desired protein. A N-terminal GST tag is fused to the protein. The recombinant Human ERCC3 protein is subjected to affinity purification. Its purity is over 85%, as measured by SDS-PAGE.
| Cat.No | ACP01209 | Target Name | ERCC3 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 1-782aa | Mol Weight | 115.6 kDa |
| Protein Length | Full length | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P19447 |
|---|
Uniprot Id
P19447
Target Species
Human
Target Name
ERCC3
Target Full Name
General transcription and DNA repair factor IIH helicase/translocase subunit XPB
Target Function
ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
Target Involvement
Xeroderma pigmentosum complementation group B (XP-B); Trichothiodystrophy 2, photosensitive (TTD2)
Target Subcellular Location
Nucleus.
Target Protein Families
Helicase family, RAD25/XPB subfamily
Target Research Area
Others
Target Synonyms
Basic transcription factor 2 89 kDa subunit; BTF 2; BTF2; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; ERCC 3; ercc3; ERCC3_HUMAN; Excision Repair Cross-complementing Rodent Repair deficiency complementation Group 3; GTF2H; RAD 25; RAD25; TFIIH 89 kDa subunit; TFIIH; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH p89; Xeroderma pigmentosum group B-complementing protein
Target Background
This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.
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