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Recombinant Human Cytidine deaminase (CDA)

The recombinant Human CDA protein is made by following sequential steps: inserting a DNA fragment encoding the Human CDA protein (1-146aa) into a plasmid vector, transferring this vector into e.coli cells, selecting the positive cells, culturing positive cells, inducing the protein expression, and collecting the CDA protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant CDA protein is subjected to affinity purification and SDS-PAGE analysis. Its purity is over 85%.

ACP01994

The recombinant Human CDA protein is made by following sequential steps: inserting a DNA fragment encoding the Human CDA protein (1-146aa) into a plasmid vector, transferring this vector into e.coli cells, selecting the positive cells, culturing positive cells, inducing the protein expression, and collecting the CDA protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant CDA protein is subjected to affinity purification and SDS-PAGE analysis. Its purity is over 85%.

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Specifications


Cat.No ACP01994 Target NameCDA
Target SynonymsCytidine aminohydrolaseFormLiquid or Lyophilized powder
Expression SystemE.coliExpression Range1-146aa
Mol Weight23.6 kDaProtein LengthFull length
PurityGreater than 85% as determined by SDS-PAGE.Storage Buffer5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Immunogen Information


Target SpeciesHumanUniprot IDP32320
Background Information
  • Uniprot Id

    P32320

  • Target Species

    Human

  • Target Name

    CDA

  • Target Full Name

    Cytidine deaminase

  • Target Function

    This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.

  • Target Protein Families

    Cytidine and deoxycytidylate deaminase family

  • Target Tissue Specificity

    Highly expressed in granulocytes while expression is very low in fibroblasts, chondrocytes, monocytes, and T- as well as B-cell lines.

  • Target Research Area

    Epigenetics and Nuclear Signaling

  • Target Synonyms

    CDA; CDDCytidine deaminase; EC 3.5.4.5; Cytidine aminohydrolase

  • Target Background

    This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias.

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