Recombinant Human Keratin, type I cuticular Ha5 (KRT35)

Synthesizing the recombinant Human KRT35 protein generally involves integrating the DNA fragment that encodes the Human KRT35 protein (1-455aa) into a plasmid, introducing the recombinant plasmid into e.coli cells, followed by the selection and culturing of positive e.coli cells, induction of protein expression, and subsequent cell lysis. A N-terminal 6xHis-SUMO tag is fused to the protein. The protein is purified through affinity purification, and SDS-PAGE analysis is conducted to confirm the presence of the protein and determine its purity. The protein's purity surpasses 90%.