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In e.coli cells, the generation of recombinant Human HAGH protein involves cloning a DNA fragment encoding the Human HAGH protein (50-308aa) into a plasmid vector, which is then transferred into the e.coli cells. Positive cells are selected, cultured, and induced to express the HAGH protein. A N-terminal GST tag is attached to the protein. Lysis of the cells allows for the harvest of the recombinant Human HAGH protein. The collected recombiant Human HAGH protein is subjected to affinity purification and is identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. Its purity is greater than 90%.
In e.coli cells, the generation of recombinant Human HAGH protein involves cloning a DNA fragment encoding the Human HAGH protein (50-308aa) into a plasmid vector, which is then transferred into the e.coli cells. Positive cells are selected, cultured, and induced to express the HAGH protein. A N-terminal GST tag is attached to the protein. Lysis of the cells allows for the harvest of the recombinant Human HAGH protein. The collected recombiant Human HAGH protein is subjected to affinity purification and is identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. Its purity is greater than 90%.
| Cat.No | ACP02872 | Target Name | HAGH |
|---|---|---|---|
| Target Synonyms | HAGH; GLO2; HAGH1Hydroxyacylglutathione hydrolase; mitochondrial; EC 3.1.2.6; Glyoxalase II; Glx II | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 50-308aa |
| Mol Weight | 55.7kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q16775 |
|---|
Uniprot Id
Q16775
Target Species
Human
Target Name
HAGH
Target Full Name
Hydroxyacylglutathione hydrolase, mitochondrial
Target Function
Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.
Target Subcellular Location
[Isoform 1]: Mitochondrion matrix.; [Isoform 2]: Cytoplasm.
Target Protein Families
Metallo-beta-lactamase superfamily, Glyoxalase II family
Target Tissue Specificity
Expressed in liver and kidney.
Target Research Area
Metabolism
Target Synonyms
HAGH; GLO2; HAGH1Hydroxyacylglutathione hydrolase; mitochondrial; EC 3.1.2.6; Glyoxalase II; Glx II
Target Background
The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene.
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