Recombinant Human Exonuclease V (EXO5)

Uniprot Id

Q9H790

Target Species

Human

Target Name

EXO5

Target Full Name

Exonuclease V

Target Function

Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity.

Target Subcellular Location

Nucleus. Cytoplasm, cytosol. Note=Localizes to repair foci in response to DNA damage.

Target Protein Families

EXO5 family

Target Research Area

Signal Transduction

Target Synonyms

EXO5; C1orf176; DEM1; Exonuclease V; Exo V; hExo5; EC 3.1.-.-; Defects in morphology protein 1 homolog

Target Background

The protein encoded by this gene is a single-stranded DNA (ssDNA)-specific exonuclease that can slide along the DNA before cutting it. However, human replication protein A binds ssDNA and restricts sliding of the encoded protein, providing a 5'-directionality to the enzyme. This protein localizes to nuclear repair loci after DNA damage.