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To prepare this Recombinant Human CASP3 protein, the recombinant DNA was required, which was generated by fusing the CASP3 gene with N-terminal 6xHis tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, E.coli, was needed for this CASP3 protein production. After purification, a premium CASP3 recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 85%+. CASP3 is a gene encoding a protein named caspase-3 (short name is CASP3) or cysteine protease CPP32 (short name is CPP32). CASP3 protein, a member of asparate-specific cysteinyl proteases family, is a small protein consists of two subunits of 12 kDa and 17 kDa. Recently, it has attracted much attention with its incredible roles in tissue differentiation, regeneration, and neural development. This enzyme is a key zymogen in cell apoptosis and is not activated until it is cleaved by initiator caspases during apoptotic flux. Except apoptosis, tt has been found involved in other biological processes, such as anterior neural tube closure, T cell homeostasis and neuron differentiation.
To prepare this Recombinant Human CASP3 protein, the recombinant DNA was required, which was generated by fusing the CASP3 gene with N-terminal 6xHis tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, E.coli, was needed for this CASP3 protein production. After purification, a premium CASP3 recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 85%+. CASP3 is a gene encoding a protein named caspase-3 (short name is CASP3) or cysteine protease CPP32 (short name is CPP32). CASP3 protein, a member of asparate-specific cysteinyl proteases family, is a small protein consists of two subunits of 12 kDa and 17 kDa. Recently, it has attracted much attention with its incredible roles in tissue differentiation, regeneration, and neural development. This enzyme is a key zymogen in cell apoptosis and is not activated until it is cleaved by initiator caspases during apoptotic flux. Except apoptosis, tt has been found involved in other biological processes, such as anterior neural tube closure, T cell homeostasis and neuron differentiation.
| Cat.No | ACP09214 | Target Name | CASP3 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 29-175 | Mol Weight | 20.6kDa |
| Protein Length | Partial | Purity | >85% (SDS-PAGE) |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P42574 |
|---|
Uniprot Id
P42574
Target Species
Human
Target Name
CASP3
Target Full Name
Caspase-3
Target Function
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
Target Subcellular Location
Cytoplasm.
Target Protein Families
Peptidase C14A family
Target Tissue Specificity
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Target Research Area
Apoptosis
Target Synonyms
A830040C14Rik; Apopain; CASP 3; CASP-3; CASP3; CASP3_HUMAN; Casp3a; Caspase 3; Caspase 3; apoptosis-related cysteine peptidase; Caspase 3; apoptosis-related cysteine protease; Caspase 3; apoptosis-related cysteine protease a; Caspase-3 subunit p12; Caspase3; CC3; CPP 32; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; ICE3; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; Protein Yama; SCA 1; SCA-1; SCA1; SREBP cleavage activity 1; Yama; Yama protein
Target Background
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
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