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The antibody against EIF5 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-235 of human EIF5 (NP_892116.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
The antibody against EIF5 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-235 of human EIF5 (NP_892116.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
| Cat.No | ADA-09462A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | EIF5 |
| Target Synonyms | EIF-5; EIF-5A; EIF5 | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | HeLa, MCF7, SW480, U-251MG | Application | ELISA, WB, IF/ICC |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-235 of human EIF5 (NP_892116.2). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | P55010 | Immunogen Sequence |
Uniprot Id
P55010
Target Species
Human
Target Name
EIF5
Target Full Name
Eukaryotic translation initiation factor 5
Target Function
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Target Protein Families
EIF-2-beta/eIF-5 family
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
2810011H21Rik; D12Ertd549e; EIF 5; EIF 5A; eIF-5; Eif5; Eukaryotic initiation factor 5; Eukaryotic translation initiation factor 5; IF5_HUMAN; MGC36374; MGC36509
Target Background
Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).
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