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The antibody against PEX14 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 118-377 of human PEX14 (NP_004556.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
The antibody against PEX14 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 118-377 of human PEX14 (NP_004556.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
Western blot analysis of Mouse kidney, using PEX14 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
| Cat.No | ADA-11041A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | PEX14 |
| Target Synonyms | NAPP2; PBD13A; Pex14p; dJ734G22.2; PEX14 | Form | Liquid |
| Species Reactivity | Human, Mouse | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Mouse kidney, A-431 | Application | ELISA, WB, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 118-377 of human PEX14 (NP_004556.1). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | O75381 | Immunogen Sequence |
Uniprot Id
O75381
Target Species
Human
Target Name
PEX14
Target Full Name
Peroxisomal membrane protein PEX14
Target Function
Peroxisome membrane protein that is an essential component of the peroxisomal import machinery. Functions as a docking factor for the predominantly cytoplasmic PTS1 receptor (PEX5). Plays a key role for peroxisome movement through a direct interaction with tubulin.
Target Involvement
Peroxisome biogenesis disorder complementation group K (PBD-CGK); Peroxisome biogenesis disorder 13A (PBD13A)
Target Subcellular Location
Peroxisome membrane; Peripheral membrane protein; Cytoplasmic side.
Target Protein Families
Peroxin-14 family
Target Synonyms
dJ734G22.2; NAPP2 ; NF E2 associated polypeptide 2 ; Peroxin 14 ; Peroxin-14; Peroxisomal biogenesis factor 14; Peroxisomal membrane anchor protein PEX14; Peroxisomal membrane anchor protein Pex14p; Peroxisomal membrane protein PEX14; pex14; PEX14_HUMAN; Pex14p; PTS1 receptor docking protein; PTS1 receptor-docking protein
Target Background
This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome.
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