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The antibody against TIPARP was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 441-657 of human TIPARP (NP_056323.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against TIPARP was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 441-657 of human TIPARP (NP_056323.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
Western blot analysis of various lysates, using TIPARP Rabbit pAb at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
| Cat.No | ADA-10218A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | TIPARP |
| Target Synonyms | PARP7; ARTD14; pART14; TIPARP | Form | Liquid |
| Species Reactivity | Human, Mouse | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.01% thimerosal, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | K-562, U-87 MG | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 441-657 of human TIPARP (NP_056323.2). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q7Z3E1 | Immunogen Sequence |
Uniprot Id
Q7Z3E1
Target Species
Human
Target Name
TIPARP
Target Full Name
Protein mono-ADP-ribosyltransferase TIPARP
Target Function
ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate, aspartate and cysteine residues on target proteins. Acts as a negative regulator of AHR by mediating mono-ADP-ribosylation of AHR, leading to inhibit transcription activator activity of AHR.
Target Subcellular Location
Nucleus.
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
ADP-ribosyltransferase diphtheria toxin-like 14; ARTD14; AW558171; DDF1; DKFZp434J214; DKFZp686N0351; DKFZp686P1838; FLJ40466; PARP 1; PARP 7; PARP-7; PARPT_HUMAN; pART14; Poly [ADP ribose] polymerase 7; Poly [ADP-ribose] polymerase 7; TCDD inducible poly(ADP ribose) polymerase; TCDD-inducible poly [ADP-ribose] polymerase; TIPARP
Target Background
This gene encodes a member of the poly(ADP-ribose) polymerase superfamily. Studies of the mouse ortholog have shown that the encoded protein catalyzes histone poly(ADP-ribosyl)ation and may be involved in T-cell function. Alternative splicing results in multiple transcript variants.
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