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The region for expressing recombinant Human PLCZ1 contains amino acids 1-415. This PLCZ1 protein is expected to have a theoretical molecular weight of 64.5 kDa. This PLCZ1 protein is produced using e.coli expression system. Fusion of the N-terminal 6xHis-SUMO tag into the PLCZ1 encoding gene fragment was conducted, allowing for easier detection and purification of the PLCZ1 protein in subsequent stages.Human 1-Phosphatidylinositol 4,5-Bisphosphate Phosphodiesterase Zeta-1 (PLCZ1) is a key enzyme involved in sperm function. Its primary function is to hydrolyze phosphatidylinositol 4,5-bisphosphate (PIP2) to generate inositol trisphosphate (IP3), initiating calcium release during sperm-egg fusion. In reproductive research, PLCZ1 is pivotal for understanding fertilization mechanisms, and its dysfunction may contribute to male infertility. Additionally, PLCZ1's role in intracellular signaling implicates it in broader cell biology and signal transduction studies. Investigating PLCZ1 provides insights into reproductive processes, male infertility, and signaling cascades, offering potential applications in assisted reproductive technologies and advancing the understanding of cellular mechanisms related to fertility and intracellular communication.
The region for expressing recombinant Human PLCZ1 contains amino acids 1-415. This PLCZ1 protein is expected to have a theoretical molecular weight of 64.5 kDa. This PLCZ1 protein is produced using e.coli expression system. Fusion of the N-terminal 6xHis-SUMO tag into the PLCZ1 encoding gene fragment was conducted, allowing for easier detection and purification of the PLCZ1 protein in subsequent stages.Human 1-Phosphatidylinositol 4,5-Bisphosphate Phosphodiesterase Zeta-1 (PLCZ1) is a key enzyme involved in sperm function. Its primary function is to hydrolyze phosphatidylinositol 4,5-bisphosphate (PIP2) to generate inositol trisphosphate (IP3), initiating calcium release during sperm-egg fusion. In reproductive research, PLCZ1 is pivotal for understanding fertilization mechanisms, and its dysfunction may contribute to male infertility. Additionally, PLCZ1’s role in intracellular signaling implicates it in broader cell biology and signal transduction studies. Investigating PLCZ1 provides insights into reproductive processes, male infertility, and signaling cascades, offering potential applications in assisted reproductive technologies and advancing the understanding of cellular mechanisms related to fertility and intracellular communication.
| Cat.No | ACP04237 | Target Name | PLCZ1 |
|---|---|---|---|
| Target Synonyms | 1 phosphatidylinositol 4 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-415aa |
| Mol Weight | 64.5kDa | Protein Length | Full Length of Isoform 2 |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q86YW0 |
|---|
Uniprot Id
Q86YW0
Target Species
Human
Target Name
PLCZ1
Target Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
Target Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
Target Involvement
Spermatogenic failure 17 (SPGF17)
Target Subcellular Location
Nucleus. Cytoplasm, perinuclear region.
Target Tissue Specificity
Expressed specifically in testis and sperm. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.
Target Synonyms
1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase zeta 1; 1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase zeta-1; MGC149685; NYD SP27; Phosphoinositide phospholipase C zeta 1; Phosphoinositide phospholipase C-zeta-1; Phospholipase C zeta 1; Phospholipase C-zeta-1; PI phospholipase C zeta 1; PLC zeta 1; PLC-zeta-1; PLCZ1; PLCZ1_HUMAN; PLCzeta; Testis development protein NYD SP27; Testis development related NYD SP27; Testis-development protein NYD-SP27
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