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The production of this Recombinant Human HSD11B1 protein started with the HSD11B1 gene synthesis. And then using recombinant DNA technology, the HSD11B1 gene was inserted into an expression vector so that we could get the recombinant express plasmid of HSD11B1. Transform the plasmid into the cells of E.coli, culture the cells and we could get the desired Recombinant Human HSD11B1 protein. But the work was not completed, protein purification and a strict QC system were performed in the last step. The purity is 90%+ determined by SDS-PAGE. HSD11B1 is a gene providing an instruction of making a protein named Corticosteroid 11-beta-dehydrogenase isozyme 1 (HSD11B1) in human. The protein encoded by this gene is also known as 11-beta-hydroxysteroid dehydrogenase 1 (11-DH or 11-beta-HSD1) and short chain dehydrogenase/reductase family 26C member 1. The encoded protein is involved in lung development and steroid catabolic process with 11-beta-hydroxysteroid dehydrogenase (NADP+) and protein homodimerization activity. Moreover, it can bind to NADP and steroid.
The production of this Recombinant Human HSD11B1 protein started with the HSD11B1 gene synthesis. And then using recombinant DNA technology, the HSD11B1 gene was inserted into an expression vector so that we could get the recombinant express plasmid of HSD11B1. Transform the plasmid into the cells of E.coli, culture the cells and we could get the desired Recombinant Human HSD11B1 protein. But the work was not completed, protein purification and a strict QC system were performed in the last step. The purity is 90%+ determined by SDS-PAGE.
HSD11B1 is a gene providing an instruction of making a protein named Corticosteroid 11-beta-dehydrogenase isozyme 1 (HSD11B1) in human. The protein encoded by this gene is also known as 11-beta-hydroxysteroid dehydrogenase 1 (11-DH or 11-beta-HSD1) and short chain dehydrogenase/reductase family 26C member 1. The encoded protein is involved in lung development and steroid catabolic process with 11-beta-hydroxysteroid dehydrogenase (NADP+) and protein homodimerization activity. Moreover, it can bind to NADP and steroid.
| Cat.No | ACP04364 | Target Name | HSD11B1 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 25-292aa | Mol Weight | 45.5kDa |
| Protein Length | Partial | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P28845 |
|---|
Uniprot Id
P28845
Target Species
Human
Target Name
HSD11B1
Target Full Name
11-beta-hydroxysteroid dehydrogenase 1
Target Function
Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
Target Involvement
Cortisone reductase deficiency 2 (CORTRD2)
Target Subcellular Location
Endoplasmic reticulum membrane; Single-pass type II membrane protein.
Target Protein Families
Short-chain dehydrogenases/reductases (SDR) family
Target Tissue Specificity
Widely expressed. Highest expression in liver.
Target Synonyms
11 beta HSD 1; 11 beta HSD1 ; 11 beta hydroxysteroid dehydrogenase 1; 11 DH ; 11-beta hydroxysteroid dehydrogenase; type 1; 11-beta-HSD1; 11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11DH ; Corticosteroid 11 beta dehydrogenase isozyme 1; Corticosteroid 11-beta-dehydrogenase isozyme 1; CORTRD2; DHI1_HUMAN; HDL; HSD 11; HSD11; HSD11B; HSD11B1; HSD11L; Hydroxysteroid (11 beta) dehydrogenase ; Hydroxysteroid (11 beta) dehydrogenase 1; MGC13539; SDR26C1; short chain dehydrogenase/reductase family 26C; member 1
Target Background
The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.
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