Recombinant Human Actin-like protein 8 (ACTL8)

Insertion of the gene encoding the Human ACTL8 protein (1-366aa) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into baculovirus cells. Positive baculovirus cells are selected relying on their ability to survive in the presence of a specific antibiotic. The baculovirus cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal Myc tag is attached to the protein. Following expression, the recombinant Human ACTL8 protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human ACTL8 protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 85%.