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The expression vector recombined with the recombinant DNA was transfected into the yeast cells for expression. The recombinant DNA resulted from the fusion of the gene coding for the 24-399aa of the human AADAC protein and the N-terminal His tag gene. The product was purified and isolated to get the recombinant human AADAC protein with N-terminal His tag. This recombinant AADAC protein's purity reaches up to 85% determined by SAS-PAGE.AADAC is a gene encoding a protein named arylacetamide deacetylase(abbreviated AADAC) and belongs to 'GDXG' lipolytic enzyme family. AADAC is a single serine esterase mainly expressed in liver, small intestine, colon, adrenal gland and bladder and to a lesser extent in the pancreas and adrenal gland. This enzyme is principally involved in the hydrolysis of several drugs containing the acetyl group. A study has demonstrated that AADAC is the principal enzyme involved in phenacetin hydrolysis with respect to CES enzyme contribution. AADAC may hydrolyze N-acetylarylamine drugs producing arylamine, which could cause toxic effects. Similar to the CES enzymes, AADAC also may be relevant in the preclinical development of inhaled drugs.
The expression vector recombined with the recombinant DNA was transfected into the yeast cells for expression. The recombinant DNA resulted from the fusion of the gene coding for the 24-399aa of the human AADAC protein and the N-terminal His tag gene. The product was purified and isolated to get the recombinant human AADAC protein with N-terminal His tag. This recombinant AADAC protein’s purity reaches up to 85% determined by SAS-PAGE.AADAC is a gene encoding a protein named arylacetamide deacetylase(abbreviated AADAC) and belongs to ‘GDXG’ lipolytic enzyme family. AADAC is a single serine esterase mainly expressed in liver, small intestine, colon, adrenal gland and bladder and to a lesser extent in the pancreas and adrenal gland. This enzyme is principally involved in the hydrolysis of several drugs containing the acetyl group. A study has demonstrated that AADAC is the principal enzyme involved in phenacetin hydrolysis with respect to CES enzyme contribution. AADAC may hydrolyze N-acetylarylamine drugs producing arylamine, which could cause toxic effects. Similar to the CES enzymes, AADAC also may be relevant in the preclinical development of inhaled drugs.
| Cat.No | ACP23775 | Target Name | AADAC |
|---|---|---|---|
| Target Synonyms | AAAD_HUMAN; Aada; Aadac; Arylacetamide deacetylase (esterase); Arylacetamide deacetylase; CES5A1; DAC | Form | Liquid or Lyophilized powder |
| Expression System | Yeast | Protein Length | Partial |
| Purity | >85% (SDS-PAGE) | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P22760 |
|---|
Uniprot Id
P22760
Target Species
Human
Target Name
AADAC
Target Full Name
Arylacetamide deacetylase
Target Function
Displays cellular triglyceride lipase activity in liver, increases the levels of intracellular fatty acids derived from the hydrolysis of newly formed triglyceride stores and plays a role in very low-density lipoprotein assembly. Displays serine esterase activity in liver. Deacetylates a variety of arylacetamide substrates, including xenobiotic compounds and procarcinogens, converting them to the primary arylamide compounds and increasing their toxicity.
Target Subcellular Location
Endoplasmic reticulum membrane; Single-pass type II membrane protein. Microsome membrane; Single-pass type II membrane protein.
Target Protein Families
'GDXG' lipolytic enzyme family
Target Tissue Specificity
Detected in liver (at protein level). Mainly expressed in liver, small intestine, colon, adrenal gland and bladder.
Target Research Area
Signal Transduction
Target Synonyms
AAAD_HUMAN; Aada; Aadac; Arylacetamide deacetylase (esterase); Arylacetamide deacetylase; CES5A1; DAC
Target Background
Microsomal arylacetamide deacetylase competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens
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