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Expressing the recombinant Human DHX33 protein in e.coli cells involves inserting a DNA fragment encoding the Human DHX33 protein (1-450aa) into a plasmid vector and transferring it to the e.coli cells. Positive cells are screened, cultured, and induced to express the DHX33 protein. The protein carries a N-terminal 6xHis tag. The cells are lysed to collect the recombinant Human DHX33 protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human DHX33 protein exceeds 85%.
Expressing the recombinant Human DHX33 protein in e.coli cells involves inserting a DNA fragment encoding the Human DHX33 protein (1-450aa) into a plasmid vector and transferring it to the e.coli cells. Positive cells are screened, cultured, and induced to express the DHX33 protein. The protein carries a N-terminal 6xHis tag. The cells are lysed to collect the recombinant Human DHX33 protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human DHX33 protein exceeds 85%.
| Cat.No | ACP02635 | Target Name | DHX33 |
|---|---|---|---|
| Target Synonyms | DHX33; DDX33ATP-dependent RNA helicase DHX33; EC 3.6.4.13; DEAH box protein 33 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-450aa |
| Mol Weight | 53.7 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9H6R0 |
|---|
Uniprot Id
Q9H6R0
Target Species
Human
Target Name
DHX33
Target Full Name
ATP-dependent RNA helicase DHX33
Target Function
Implicated in nucleolar organization, ribosome biogenesis, protein synthesis and cytoplasmic dsRNA sensing. Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment. In the cytoplasm, promotes elongation-competent 80S ribosome assembly at the late stage of mRNA translation initiation. Senses cytosolic dsRNA mediating NLRP3 inflammasome formation in macrophages and type I interferon production in myeloid dendritic cells. Required for NLRP3 activation induced by viral dsRNA and bacterial RNA. In dendritic cells, required for induction of type I interferon production induced by cytoplasmic dsRNA via the activation of MAPK and NF-kappa-B signaling pathways.
Target Subcellular Location
Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm. Nucleus. Inflammasome.
Target Protein Families
DEAD box helicase family, DEAH subfamily
Target Research Area
Cell Biology
Target Synonyms
DHX33; DDX33ATP-dependent RNA helicase DHX33; EC 3.6.4.13; DEAH box protein 33
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