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Just like other recombinant proteins, the production of this recombinant Human ATP5F1B protein began with appropriate cDNA and PCR methods, and then the ATP5F1B expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into E.coli for the expression of the recombinant Human ATP5F1B protein. N-terminal 10xHis-SUMO tag & C-terminal Myc tag was used in the process. And we finally get the protein of interest with purity of 85%+.ATP5F1B (also called ATP5B, ATPMB, ATPSB) is a gene that encodes a subunit of mitochondrial ATP synthase named ATP synthase subunit beta, mitochondrial (also known as ATP synthase F1 subunit beta) in human. Mitochondrial ATP synthase (also known as complex V) consists of two functional domains, F1and Fo. F1 is situated in the mitochondrial matrix, and Fo is located in the inner mitochondrial membrane. This ATP synthase catalyzes the formation of the energy storage molecule adenosine triphosphate (ATP) with adenosine diphosphate (ADP) and inorganic phosphate (P?).
Just like other recombinant proteins, the production of this recombinant Human ATP5F1B protein began with appropriate cDNA and PCR methods, and then the ATP5F1B expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into E.coli for the expression of the recombinant Human ATP5F1B protein. N-terminal 10xHis-SUMO tag & C-terminal Myc tag was used in the process. And we finally get the protein of interest with purity of 85%+.ATP5F1B (also called ATP5B, ATPMB, ATPSB) is a gene that encodes a subunit of mitochondrial ATP synthase named ATP synthase subunit beta, mitochondrial (also known as ATP synthase F1 subunit beta) in human. Mitochondrial ATP synthase (also known as complex V) consists of two functional domains, F1and Fo. F1 is situated in the mitochondrial matrix, and Fo is located in the inner mitochondrial membrane. This ATP synthase catalyzes the formation of the energy storage molecule adenosine triphosphate (ATP) with adenosine diphosphate (ADP) and inorganic phosphate (P?).
| Cat.No | ACP02033 | Target Name | Atp5f1b |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 230-529aa | Mol Weight | 52.8 kDa |
| Protein Length | Partial | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P06576 |
|---|
Uniprot Id
P06576
Target Species
Human
Target Name
ATP5F1B
Target Full Name
ATP synthase subunit beta, mitochondrial
Target Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Target Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Target Protein Families
ATPase alpha/beta chains family
Target Research Area
Tags & Cell Markers
Target Synonyms
ATP 5B; ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide; ATP synthase subunit beta mitochondrial; ATP synthase subunit beta; mitochondrial; atp5b; ATPB; ATPB_HUMAN; ATPMB; ATPSB; Epididymis secretory protein Li 271; HEL-S-271; Mitochondrial ATP synthase beta subunit; Mitochondrial ATP Synthase Subunit Beta; Mitochondrial ATP synthetase beta subunit
Target Background
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
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