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The gene encoding the Human BMP7 protein (293-431aa) is incorporated into a plasmid vector to generate recombinant plasmid, which is then introduced into e.coli cells. The e.coli cells containing the recombinant plasmid are selected and cultured under conditions that stimulate the expression of the gene of interest. A N-terminal 6xHis tag is attached to the protein. After expression, affinity purification is employed to isolate and purify the recombinant Human BMP7 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human BMP7 protein, revealing a purity level exceeding 90%.
The gene encoding the Human BMP7 protein (293-431aa) is incorporated into a plasmid vector to generate recombinant plasmid, which is then introduced into e.coli cells. The e.coli cells containing the recombinant plasmid are selected and cultured under conditions that stimulate the expression of the gene of interest. A N-terminal 6xHis tag is attached to the protein. After expression, affinity purification is employed to isolate and purify the recombinant Human BMP7 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human BMP7 protein, revealing a purity level exceeding 90%.
| Cat.No | ACP02942 | Target Name | BMP7 |
|---|---|---|---|
| Target Synonyms | BMP 7; BMP-7; Bmp7; BMP7_HUMAN; Bone morphogenetic protein 7; Bone morphogenic protein 7; Eptotermin alfa ; OP 1; OP-1; OP1 ; Osteogenic protein 1 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 293-431aa |
| Mol Weight | 19.7kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P18075 |
|---|
Uniprot Id
P18075
Target Species
Human
Target Name
BMP7
Target Full Name
Bone morphogenetic protein 7
Target Function
Growth factor of the TGF-beta superfamily that plays important role in various biological processes, including embryogenesis, hematopoiesis, neurogenesis and skeletal morphogenesis. Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2A. Once all three components are bound together in a complex at the cell surface, ACVR2A phosphorylates and activates ACVR1. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. For specific functions such as growth cone collapse in developing spinal neurons and chemotaxis of monocytes, uses also BMPR2 as type II receptor. Can also signal through non-canonical pathways such as P38 MAP kinase signaling cascade that promotes brown adipocyte differentiation through activation of target genes, including members of the SOX family of transcription factors.
Target Subcellular Location
Secreted.
Target Protein Families
TGF-beta family
Target Tissue Specificity
Expressed in the kidney and bladder. Lower levels seen in the brain.
Target Research Area
Developmental Biology
Target Synonyms
BMP 7; BMP-7; Bmp7; BMP7_HUMAN; Bone morphogenetic protein 7; Bone morphogenic protein 7; Eptotermin alfa ; OP 1; OP-1; OP1 ; Osteogenic protein 1
Target Background
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone, kidney and brown adipose tissue development. Additionally, this protein induces ectopic bone formation and may promote fracture healing in human patients.
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