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The expression of recombinant Human CA5A protein involves transfecting mammalian cells with a DNA expression vector carrying the gene for the protein of interest (39-305aa). After transfection, the cells are cultured to produce the desired protein. The recombinant Human CA5A protein is collected and purified from the cell lysate through affinity purification, attaining a purity greater than 85%, as determined by SDS-PAGE.
The expression of recombinant Human CA5A protein involves transfecting mammalian cells with a DNA expression vector carrying the gene for the protein of interest (39-305aa). After transfection, the cells are cultured to produce the desired protein. The recombinant Human CA5A protein is collected and purified from the cell lysate through affinity purification, attaining a purity greater than 85%, as determined by SDS-PAGE.
| Cat.No | ACP01788 | Target Name | CA5A |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Mammalian cell |
| Expression Range | 39-305aa | Mol Weight | 30.6 kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P35218 |
|---|
Uniprot Id
P35218
Target Species
Human
Target Name
CA5A
Target Full Name
Carbonic anhydrase 5A, mitochondrial
Target Function
Reversible hydration of carbon dioxide. Low activity.
Target Involvement
Hyperammonemia due to carbonic anhydrase VA deficiency (CA5AD)
Target Subcellular Location
Mitochondrion.
Target Protein Families
Alpha-carbonic anhydrase family
Target Research Area
Metabolism
Target Synonyms
CA5A; CA5Carbonic anhydrase 5A; mitochondrial; EC 4.2.1.1; Carbonate dehydratase VA; Carbonic anhydrase VA; CA-VA
Target Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VA is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2.
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