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The gene encoding the Human C8G protein (21-202aa) is incorporated into a plasmid vector to generate recombinant plasmid, which is then introduced into e.coli cells. The e.coli cells containing the recombinant plasmid are selected and cultured under conditions that stimulate the expression of the gene of interest. A N-terminal 6xHis tag is attached to the protein. After expression, affinity purification is employed to isolate and purify the recombinant Human C8G protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human C8G protein, revealing a purity level exceeding 85%.
The gene encoding the Human C8G protein (21-202aa) is incorporated into a plasmid vector to generate recombinant plasmid, which is then introduced into e.coli cells. The e.coli cells containing the recombinant plasmid are selected and cultured under conditions that stimulate the expression of the gene of interest. A N-terminal 6xHis tag is attached to the protein. After expression, affinity purification is employed to isolate and purify the recombinant Human C8G protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human C8G protein, revealing a purity level exceeding 85%.
| Cat.No | ACP00017 | Target Name | C8G |
|---|---|---|---|
| Target Synonyms | C8C; C8G; CO8G_HUMAN; Complement component 8 gamma polypeptide; Complement component C8 gamma chain; MGC142186 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 21-202aa |
| Mol Weight | 26.4 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P07360 |
|---|
Uniprot Id
P07360
Target Species
Human
Target Name
C8G
Target Full Name
Complement component C8 gamma chain
Target Function
C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol.
Target Subcellular Location
Secreted.
Target Protein Families
Calycin superfamily, Lipocalin family
Target Research Area
Immunology
Target Synonyms
C8C; C8G; CO8G_HUMAN; Complement component 8 gamma polypeptide; Complement component C8 gamma chain; MGC142186
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