Recombinant Human DNA polymerase delta catalytic subunit (POLD1), Truncated

Uniprot Id

P28340

Target Species

Human

Target Name

POLD1

Target Full Name

DNA polymerase delta catalytic subunit

Target Function

As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites.

Target Involvement

Colorectal cancer 10 (CRCS10); Mandibular hypoplasia, deafness, progeroid features, and lipodystrophy syndrome (MDPL)

Target Subcellular Location

Nucleus.

Target Protein Families

DNA polymerase type-B family

Target Tissue Specificity

Widely expressed, with high levels of expression in heart and lung.

Target Synonyms

Polymerase (DNA directed) delta 1 catalytic subunit; CDC2; CDC2 homolog; CRCS10; DNA directed DNA polymerase delta 1; DNA directed polymerase delta 1; DNA pol delta 1; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125; DPOD1_HUMAN; MDPL; POLD; POLD 1; POLD1; Polymerase (DNA directed) delta 1 catalytic subunit 125kDa; Polymerase (DNA) delta 1 catalytic subunit; Polymerase DNA directed delta 1 catalytic subunit 125kD; polymerase, DNA, delta

Target Background

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.