Recombinant Human E3 ubiquitin-protein ligase TRIM32 (TRIM32)

Uniprot Id

Q13049

Target Species

Human

Target Name

TRIM32

Target Full Name

E3 ubiquitin-protein ligase TRIM32

Target Function

Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha. Also acts as a regulator of autophagy by mediating formation of unanchored 'Lys-63'-linked polyubiquitin chains that activate ULK1: interaction with AMBRA1 is required for ULK1 activation.; (Microbial infection) May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.

Target Involvement

Limb-girdle muscular dystrophy 2H (LGMD2H); Bardet-Biedl syndrome 11 (BBS11)

Target Subcellular Location

Cytoplasm.

Target Protein Families

TRIM/RBCC family

Target Tissue Specificity

Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.

Target Synonyms

72 kda Tat interacting Protein; 72 kDa Tat-interacting protein; BBS11 ; E3 ubiquitin-protein ligase TRIM32; HT2A; LGMD2H ; Limb girdle muscular dystrophy 2H (autosomal recessive); Limb girdle muscular dystrophy 2H; Muscular dystrophy Hutterite type; TAT interactive protein 72KD; TATIP ; TRI32_HUMAN; Trim32; Tripartite Motif Containing Protein 32; Tripartite motif-containing protein 32; Zinc finger protein HT2A

Target Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.