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The gene responsible for the Human HAPLN2 protein (27-340aa) is incorporated into a plasmid vector, forming recombinant plasmid. The resulting recombinant plasmid is introduced into e.coli cells, from which cells survive in the presence of a specific antibiotic are selected. The selected e.coli cells containing the recombinant plasmid are cultured under conditions that facilitate the expression of the gene of interest. A N-terminal 10xHis tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human HAPLN2 protein from the cell lysate. Denaturing SDS-PAGE is employed to resolve the resulting recombinant Human HAPLN2 protein, revealing a purity greater than 85%.
The gene responsible for the Human HAPLN2 protein (27-340aa) is incorporated into a plasmid vector, forming recombinant plasmid. The resulting recombinant plasmid is introduced into e.coli cells, from which cells survive in the presence of a specific antibiotic are selected. The selected e.coli cells containing the recombinant plasmid are cultured under conditions that facilitate the expression of the gene of interest. A N-terminal 10xHis tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human HAPLN2 protein from the cell lysate. Denaturing SDS-PAGE is employed to resolve the resulting recombinant Human HAPLN2 protein, revealing a purity greater than 85%.
| Cat.No | ACP05073 | Target Name | HAPLN2 |
|---|---|---|---|
| Target Synonyms | Brain link protein 1; Bral1; Hapln2; HPLN2_HUMAN; Hyaluronan and proteoglycan link protein 2 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 27-340aa |
| Mol Weight | 40.8 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9GZV7 |
|---|
Uniprot Id
Q9GZV7
Target Species
Human
Target Name
HAPLN2
Target Full Name
Hyaluronan and proteoglycan link protein 2
Target Function
Mediates a firm binding of versican V2 to hyaluronic acid. May play a pivotal role in the formation of the hyaluronan-associated matrix in the central nervous system (CNS) which facilitates neuronal conduction and general structural stabilization. Binds to hyaluronic acid.
Target Subcellular Location
Secreted, extracellular space, extracellular matrix.
Target Protein Families
HAPLN family
Target Tissue Specificity
Expressed only in adult brain.
Target Research Area
Neuroscience
Target Synonyms
Brain link protein 1; Bral1; Hapln2; HPLN2_HUMAN; Hyaluronan and proteoglycan link protein 2
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