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The expression of recombinant Human IDO2 protein in Yeast cells requires incorporating a DNA fragment encoding the Human IDO2 protein (14-420aa) into a plasmid vector and transforming this vector into Yeast cells. Positive cells are screened, cultured, and induced to produce the IDO2 protein. A N-terminal 6xHis tag is attached to the protein. The recombinant Human IDO2 protein is collected and purified from the cell lysate through affinity purification. It is assessed through SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue, with a purity of exceeding 90%.
The expression of recombinant Human IDO2 protein in Yeast cells requires incorporating a DNA fragment encoding the Human IDO2 protein (14-420aa) into a plasmid vector and transforming this vector into Yeast cells. Positive cells are screened, cultured, and induced to produce the IDO2 protein. A N-terminal 6xHis tag is attached to the protein. The recombinant Human IDO2 protein is collected and purified from the cell lysate through affinity purification. It is assessed through SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue, with a purity of exceeding 90%.
| Cat.No | ACP03858 | Target Name | IDO2 |
|---|---|---|---|
| Target Synonyms | 3 dioxygenase 2; Indoleamine 2, 3 dioxygenase like 1 protein ; Indoleamine 2, 3 dioxygenase like 1; Indoleamine pyrrole 2, 3 dioxygenase like protein 1; Indoleamine pyrrole 2, 3-dioxygenase 2; 3-dioxygenase-like protein 1; EC 1.13.11.; I23O2_HUMAN; IDO 2; IDO-2; Ido2; INDOL 1; Indoleamine 2; Indoleamine 2 | Form | Liquid or Lyophilized powder |
| Expression System | Yeast | Expression Range | 1-407aa |
| Mol Weight | 50.4kDa | Protein Length | Full length |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q6ZQW0 |
|---|
Uniprot Id
Q6ZQW0
Target Species
Human
Target Name
IDO2
Target Full Name
Indoleamine 2,3-dioxygenase 2
Target Function
Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in immune regulation. May not play a significant role in tryptophan-related tumoral resistance.
Target Protein Families
Indoleamine 2,3-dioxygenase family
Target Tissue Specificity
Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. Also expressed at low level in testis and thyroid. Not expressed in the majority of human tumor samples (>99%).
Target Research Area
Metabolism
Target Synonyms
3-dioxygenase 2; 3-dioxygenase-like protein 1; EC 1.13.11.; I23O2_HUMAN; IDO 2; IDO-2; Ido2; INDOL 1; Indoleamine 2; Indoleamine 2,3 dioxygenase 2; Indoleamine 2,3 dioxygenase like 1 protein ; Indoleamine 2,3 dioxygenase like protein 1; Indoleamine pyrrole 2,3 dioxygenase like 1; Indoleamine pyrrole 2,3 dioxygenase like protein 1; Indoleamine-pyrrole 2
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