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The process for producing recombinant Human EBI3 protein in e.coli cells follows a sequence of steps: creating the expression vector that codes for the Human EBI3 protein (21-227aa), introducing it into e.coli cells, cultivating and inducing of positive cells for protein expression, lysing cells, and conducting expression analysis. The resultant recombinant Human EBI3 protein is purified from the cell lysate using affinity purification, with its purity exceeding 85%, as determined by SDS-PAGE.
The process for producing recombinant Human EBI3 protein in e.coli cells follows a sequence of steps: creating the expression vector that codes for the Human EBI3 protein (21-227aa), introducing it into e.coli cells, cultivating and inducing of positive cells for protein expression, lysing cells, and conducting expression analysis. The resultant recombinant Human EBI3 protein is purified from the cell lysate using affinity purification, with its purity exceeding 85%, as determined by SDS-PAGE.
| Cat.No | ACP01954 | Target Name | EBI3 |
|---|---|---|---|
| Target Synonyms | IL-27 subunit beta;IL-27B;Epstein-Barr virus-induced gene 3 protein homolog | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 21-227aa |
| Mol Weight | 54.6 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q14213 |
|---|
Uniprot Id
Q14213
Target Species
Human
Target Name
EBI3
Target Full Name
Interleukin-27 subunit beta
Target Function
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
Target Subcellular Location
Secreted.
Target Protein Families
Type I cytokine receptor family, Type 3 subfamily
Target Research Area
Immunology
Target Synonyms
IL-27 subunit beta;IL-27B;Epstein-Barr virus-induced gene 3 protein homolog
Target Background
This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells.
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