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The production of recombinant Human MELTF protein involves manipulating the expression of the desired gene in e.coli cells. The sequence (20-302aa) of foreign DNA is merged with an expression vector and subsequently introduced into e.coli cells. Positive cells are chosen and cultivated to induce the expression of the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The resulting recombinant Human MELTF protein undergoes purification through affinity purification, with a purity exceeding 85%, as confirmed by SDS-PAGE.
The production of recombinant Human MELTF protein involves manipulating the expression of the desired gene in e.coli cells. The sequence (20-302aa) of foreign DNA is merged with an expression vector and subsequently introduced into e.coli cells. Positive cells are chosen and cultivated to induce the expression of the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The resulting recombinant Human MELTF protein undergoes purification through affinity purification, with a purity exceeding 85%, as confirmed by SDS-PAGE.
| Cat.No | ACP01212 | Target Name | MELTF |
|---|---|---|---|
| Target Synonyms | (Melanoma-associated antigen p97)(CD antigen CD228) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 20-302aa |
| Mol Weight | 35.7 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P08582 |
|---|
Uniprot Id
P08582
Target Species
Human
Target Name
MELTF
Target Full Name
Melanotransferrin
Target Function
Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.
Target Subcellular Location
[Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.
Target Protein Families
Transferrin family
Target Tissue Specificity
Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.
Target Research Area
Others
Target Synonyms
Antigen p97; CD228; CD228 antigen; MAP97 ; Melanoma associated antigen p97; Melanoma-associated antigen p97; Melanotransferrin; Mfi2; MTF1 ; TRFM_HUMAN
Target Background
The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.
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