Recombinant Human N (G),N (G)-dimethylarginine dimethylaminohydrolase 2 (DDAH2)

The process of expressing the recombinant Human DDAH2 protein includes transfecting e.coli cells with a DNA expression vector carrying the gene for the desired protein (1-285aa). Subsequently, the cells are cultured to induce the expression of the intended protein. The protein is equipped with a N-terminal 6xHis-GST tag. The recombinant Human DDAH2 protein is then collected and purified from the cell lysate through affinity purification, exhibiting a purity exceeding 85%, as verified by SDS-PAGE.

ACP01704

Number

Cat.No	ACP01704	Target Name	DDAH2
Target Synonyms	(DDAH-2)(Dimethylarginine dimethylaminohydrolase 2)(DDAHII)(Dimethylargininase-2)(Protein G6a)(S-phase protein)	Form	Liquid or Lyophilized powder
Expression System	E.coli	Expression Range	1-285aa
Mol Weight	61.2 kDa	Protein Length	Full length
Purity	Greater than 85% as determined by SDS-PAGE.	Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP01704

Target Name

DDAH2

Target Synonyms

(DDAH-2)(Dimethylarginine dimethylaminohydrolase 2)(DDAHII)(Dimethylargininase-2)(Protein G6a)(S-phase protein)

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

1-285aa

Mol Weight

61.2 kDa

Protein Length

Full length

Purity

Greater than 85% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	O95865

Background Information

Uniprot Id

O95865
Target Species

Human
Target Name

DDAH2
Target Full Name

Putative hydrolase DDAH2
Target Function

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Target Subcellular Location

Cytoplasm. Mitochondrion. Note=Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes.
Target Protein Families

DDAH family
Target Tissue Specificity

Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.
Target Research Area

Neuroscience
Target Synonyms

DDAH; DDAH II; DDAH-2; DDAH2; DDAH2_HUMAN; DDAHII; Dimethylargininase 2; Dimethylargininase-2; Dimethylarginine dimethylaminohydrolase 2; Dimethylarginine dimethylaminohydrolase II; G6a; N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; NG dimethylarginine dimethylamino hydrolase homolog; NG30; OTTHUMP00000029307; OTTHUMP00000029310; OTTHUMP00000174488; OTTHUMP00000174489; Protein G6a; S phase protein; S-phase protein
Target Background

This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.

Recombinant Human N (G),N (G)-dimethylarginine dimethylaminohydrolase 2 (DDAH2)

Specifications

Immunogen Information

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