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The production of the recombinant Human RNASE2 protein begins with the creation of the recombinant plasmid, which is synthesized by inserting the gene encoding the Human RNASE2 protein (28-161aa) into a plasmid vector. The recombinant plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected and then cultured under conditions conducive to the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis tag. Following expression, the recombinant RNASE2 protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is then employed to resolve the resulting recombinant Human RNASE2 protein, demonstrating a purity exceeding 90%.
The production of the recombinant Human RNASE2 protein begins with the creation of the recombinant plasmid, which is synthesized by inserting the gene encoding the Human RNASE2 protein (28-161aa) into a plasmid vector. The recombinant plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected and then cultured under conditions conducive to the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis tag. Following expression, the recombinant RNASE2 protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is then employed to resolve the resulting recombinant Human RNASE2 protein, demonstrating a purity exceeding 90%.
| Cat.No | ACP04303 | Target Name | RNASE2 |
|---|---|---|---|
| Target Synonyms | RNASE2; EDN; RNS2; Non-secretory ribonuclease; EC 4.6.1.18; Eosinophil-derived neurotoxin; RNase UpI-2; Ribonuclease 2; RNase 2; Ribonuclease US | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 28-161aa |
| Mol Weight | 19.5kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P10153 |
|---|
Uniprot Id
P10153
Target Species
Human
Target Name
RNASE2
Target Full Name
Non-secretory ribonuclease
Target Function
This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities.
Target Subcellular Location
Lysosome. Cytoplasmic granule. Note=Matrix of eosinophil's large specific granule.
Target Protein Families
Pancreatic ribonuclease family
Target Tissue Specificity
Liver, lung, spleen, leukocytes and body fluids.
Target Research Area
Immunology
Target Synonyms
RNASE2; EDN; RNS2; Non-secretory ribonuclease; EC 4.6.1.18; Eosinophil-derived neurotoxin; RNase UpI-2; Ribonuclease 2; RNase 2; Ribonuclease US
Target Background
The protein encoded by this gene is a non-secretory ribonuclease that belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein antimicrobial activity against viruses.
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