Recombinant?Human?Protein?argonaute-2 (AGO2), Truncated

Amino acids 517-818 constitute the expression domain of recombinant Human AGO2. This AGO2 protein is expected to have a theoretical molecular weight of 38.1 kDa. Expression of this AGO2 protein is conducted in e.coli. The N-terminal 6xHis tag was fused into the coding gene segment of AGO2, making it easier to detect and purify the AGO2 recombinant protein in the later stages of expression and purification.The human protein argonaute-2 (AGO2) is a key component of the RNA-induced silencing complex (RISC) and a member of the Argonaute protein family. AGO2 plays a central role in RNA interference (RNAi) and microRNA (miRNA) pathways. AGO2 is involved in post-transcriptional gene regulation by binding to small RNA molecules, such as miRNAs and small interfering RNAs (siRNAs). This binding allows AGO2 to guide RISC to complementary mRNA sequences, leading to mRNA cleavage or translational repression. AGO2 is essential for processes like gene silencing, antiviral defense, and maintaining genome stability. Research on AGO2 explores its intricate functions in RNA silencing pathways and its potential applications in therapeutic interventions.

ACP04031

Number

Cat.No	ACP04031	Target Name	AGO2
Form	Liquid or Lyophilized powder	Expression System	E.coli
Expression Range	517-818aa	Mol Weight	38.1kDa
Protein Length	Partial	Purity	Greater than 90% as determined by SDS-PAGE.
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP04031

Target Name

AGO2

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

517-818aa

Mol Weight

38.1kDa

Protein Length

Partial

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	Q9UKV8

Background Information

Uniprot Id

Q9UKV8
Target Species

Human
Target Name

AGO2
Target Full Name

Protein argonaute-2
Target Function

Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Target Subcellular Location

Cytoplasm, P-body. Nucleus. Note=Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.
Target Protein Families

Argonaute family, Ago subfamily
Target Research Area

Epigenetics and Nuclear Signaling
Target Synonyms

Ago 2; AGO2_HUMAN; Argonaute 2; argonaute 2; RISC catalytic component; Argonaute RISC catalytic component 2; Argonaute2; CTA-204B4.6; dAgo2; eIF 2C 2; eIF-2C 2; eIF2C 2; Eif2c2; Eukaryotic translation initiation factor 2C 2; Eukaryotic translation initiation factor 2C subunit 2; hAgo2; MGC3183; PAZ Piwi domain protein; PPD; Protein argonaute-2; Protein slicer; Q10; Slicer protein
Target Background

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.

Recombinant?Human?Protein?argonaute-2 (AGO2), Truncated

Specifications

Immunogen Information

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