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An appropriate expression vector successfully incorporates the full-length human PPP1R1B gene fragment, spanning amino acids 1-168 and featuring an N-terminal 6xHis-tag. Subsequently, the recombinant expression vector harboring the PPP1R1B gene fragment is introduced into competent E. coli cells. Following a meticulous selection process, the transformed cells are cultured to enable the expression of the desired recombinant protein. After a suitable induction period, the E. coli cells are harvested and lysed, resulting in the release of cellular contents, including the expressed recombinant PPP1R1B protein. SDS-PAGE analysis reveals the purity of the obtained human PPP1R1B protein to surpass 90%.
An appropriate expression vector successfully incorporates the full-length human PPP1R1B gene fragment, spanning amino acids 1-168 and featuring an N-terminal 6xHis-tag. Subsequently, the recombinant expression vector harboring the PPP1R1B gene fragment is introduced into competent E. coli cells. Following a meticulous selection process, the transformed cells are cultured to enable the expression of the desired recombinant protein. After a suitable induction period, the E. coli cells are harvested and lysed, resulting in the release of cellular contents, including the expressed recombinant PPP1R1B protein. SDS-PAGE analysis reveals the purity of the obtained human PPP1R1B protein to surpass 90%.
| Cat.No | ACP01035 | Target Name | PPP1R1B |
|---|---|---|---|
| Target Synonyms | (DARPP-32)(Dopamine- and cAMP-regulated neuronal phosphoprotein) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-168aa |
| Mol Weight | 23.2 kDa | Protein Length | Full Length of Isoform 2 |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9UD71 |
|---|
Uniprot Id
Q9UD71
Target Species
Human
Target Name
PPP1R1B
Target Full Name
Protein phosphatase 1 regulatory subunit 1B
Target Function
Inhibitor of protein-phosphatase 1.
Target Subcellular Location
Cytoplasm.
Target Protein Families
Protein phosphatase inhibitor 1 family
Target Research Area
Neuroscience
Target Synonyms
DARPP 32; DARPP-32; Dopamine and cAMP regulated neuronal phosphoprotein 32; Dopamine and cAMP regulated neuronal phosphoprotein; Dopamine and cAMP regulated phosphoprotein; Dopamine and cAMP regulated phosphoprotein DARPP 32 ; Dopamine and cAMP regulated phosphoprotein DARPP32; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; IPPD; Neuronal phosphoprotein DARPP 32; PPP1R1B; PPR1B_HUMAN; Protein phosphatase 1 regulatory (inhibitor) subunit 1B; Protein phosphatase 1 regulatory inhibitor subunit 1B; Protein phosphatase 1 regulatory subunit 1B
Target Background
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene.
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