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Expressing the recombinant Human RBL2 protein generally involves constructing a plasmid encoding the Human RBL2 protein (417-616aa). This plasmid is introduced into e.coli cells, followed by the selection and culturing of positive e.coli cells, induction of protein expression, and subsequent cell lysis. The protein is fused with a N-terminal 6xHis tag. The recombinant Human RBL2 protein is purified through affinity purification, and SDS-PAGE analysis is conducted to confirm the presence of the protein and determine its purity. This protein exhibits a purity exceeding 85%.
Expressing the recombinant Human RBL2 protein generally involves constructing a plasmid encoding the Human RBL2 protein (417-616aa). This plasmid is introduced into e.coli cells, followed by the selection and culturing of positive e.coli cells, induction of protein expression, and subsequent cell lysis. The protein is fused with a N-terminal 6xHis tag. The recombinant Human RBL2 protein is purified through affinity purification, and SDS-PAGE analysis is conducted to confirm the presence of the protein and determine its purity. This protein exhibits a purity exceeding 85%.
| Cat.No | ACP02139 | Target Name | RBL2 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 417-616aa | Mol Weight | 27.4 kDa |
| Protein Length | Partial | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q08999 |
|---|
Uniprot Id
Q08999
Target Species
Human
Target Name
RBL2
Target Full Name
Retinoblastoma-like protein 2
Target Function
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Target Subcellular Location
Nucleus.
Target Protein Families
Retinoblastoma protein (RB) family
Target Research Area
Cancer
Target Synonyms
130 kDa retinoblastoma associated protein; 130 kDa retinoblastoma-associated protein; IB4; p130; PPAR-alpha-interacting complex protein 128; PRB2; Rb2; Rbl2; RBL2_HUMAN; RBR 2; RBR-2; RBR2; Retinoblastoma like 2; Retinoblastoma-like protein 2; Retinoblastoma-related protein 2; RIC128
Target Background
Enables promoter-specific chromatin binding activity. Involved in regulation of lipid kinase activity. Acts upstream of or within negative regulation of gene expression. Located in chromosome; cytosol; and nuclear lumen.
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