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The generation of the recombinant Human SNAP25 protein typically includes the following steps: synthesizing the recombinant plasmid containing the gene encoding the Human SNAP25 protein (1-206aa), transforming the recombinant plasmid into e.coli cells, selecting the positive e.coli cells, and culturing the positive e.coli cells for protein expression. The protein is equipped with a N-terminal 6xHis tag. After expression, affinity purification is employed to isolate and purify the recombinant SNAP25 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human SNAP25 protein, revealing a purity level exceeding 90%.
The generation of the recombinant Human SNAP25 protein typically includes the following steps: synthesizing the recombinant plasmid containing the gene encoding the Human SNAP25 protein (1-206aa), transforming the recombinant plasmid into e.coli cells, selecting the positive e.coli cells, and culturing the positive e.coli cells for protein expression. The protein is equipped with a N-terminal 6xHis tag. After expression, affinity purification is employed to isolate and purify the recombinant SNAP25 protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human SNAP25 protein, revealing a purity level exceeding 90%.
| Cat.No | ACP04242 | Target Name | SNAP25 |
|---|---|---|---|
| Target Synonyms | 25-KD | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-206aa |
| Mol Weight | 27.3kDa | Protein Length | Full Length of Isoform 2 |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P60880 |
|---|
Uniprot Id
P60880
Target Species
Human
Target Name
SNAP25
Target Full Name
Synaptosomal-associated protein 25
Target Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Target Involvement
Myasthenic syndrome, congenital, 18 (CMS18)
Target Subcellular Location
Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Photoreceptor inner segment.
Target Protein Families
SNAP-25 family
Target Tissue Specificity
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Target Research Area
Neuroscience
Target Synonyms
bA416N4.2; Bdr; CMS18; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC-4; RIC4; SEC 9; SEC9; SNAP 25; SNAP; SNAP-25; SNAP-25B; SNAP25; SNP 25; SNP25; SNP25_HUMAN; sp; SUP; Super protein; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal associated protein 25; Synaptosomal associated protein 25kDa; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; Synaptosomal-associated protein; Synaptosomal-associated protein, 25-KD
Target Background
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
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