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The first step in producing the recombinant Human TTBK2 protein is to construct a plasmid that encodes the Human TTBK2 protein (18-287aa). The next is to transform this plasmid into e.coli cells, select positive e.coli cells, from which positive cells can be screened and cultured to express the protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The recombinant Human TTBK2 protein is purified through affinity purification from the cell lysate. Its purity is greater than 85%, determined by the SDS-PAGE analysis.
The first step in producing the recombinant Human TTBK2 protein is to construct a plasmid that encodes the Human TTBK2 protein (18-287aa). The next is to transform this plasmid into e.coli cells, select positive e.coli cells, from which positive cells can be screened and cultured to express the protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The recombinant Human TTBK2 protein is purified through affinity purification from the cell lysate. Its purity is greater than 85%, determined by the SDS-PAGE analysis.
| Cat.No | ACP05130 | Target Name | TTBK2 |
|---|---|---|---|
| Target Synonyms | TTBK2; KIAA0847; Tau-tubulin kinase 2; EC 2.7.11.1 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 18-287aa |
| Mol Weight | 38.7 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q6IQ55 |
|---|
Uniprot Id
Q6IQ55
Target Species
Human
Target Name
TTBK2
Target Full Name
Tau-tubulin kinase 2
Target Function
Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro.
Target Involvement
Spinocerebellar ataxia 11 (SCA11)
Target Subcellular Location
Cell projection, cilium. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytosol. Nucleus.
Target Protein Families
Protein kinase superfamily, CK1 Ser/Thr protein kinase family
Target Research Area
Neuroscience
Target Synonyms
TTBK2; KIAA0847; Tau-tubulin kinase 2; EC 2.7.11.1
Target Background
This gene encodes a serine-threonine kinase that putatively phosphorylates tau and tubulin proteins. Mutations in this gene cause spinocerebellar ataxia type 11 (SCA11); a neurodegenerative disease characterized by progressive ataxia and atrophy of the cerebellum and brainstem.
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