Recombinant Human Thymidylate kinase (DTYMK)

The expression region of this recombinant Human DTYMK covers amino acids 1-212. The calculated molecular weight for this DTYMK protein is 50.8 kDa. Expression of this DTYMK protein is conducted in e.coli. The DTYMK gene fragment has been modified by fusing the N-terminal GST tag, providing convenience in detecting and purifying the recombinant DTYMK protein during the following stages.Thymidylate kinase (DTYMK) is a crucial enzyme involved in nucleotide metabolism, specifically in the synthesis of DNA. It catalyzes the phosphorylation of dTMP (deoxythymidine monophosphate) to form dTDP (deoxythymidine diphosphate). This reaction is essential for the deoxythymidine triphosphate (dTTP) biosynthesis pathway, a precursor necessary for DNA replication and repair. DTYMK contributes to the maintenance of proper nucleotide pools within cells, ensuring the availability of building blocks for DNA synthesis. Its activity is tightly regulated to support genomic integrity and cellular proliferation. Understanding the function of DTYMK provides insights into the broader cellular processes involved in DNA replication and repair, offering potential targets for therapeutic interventions in conditions where nucleotide metabolism is dysregulated.