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Recombination of a plasmid encoding the Human UCHL5 protein (1-326aa) is the first step during the production the recombinant Human UCHL5 protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal 6xHis-SUMO tag. After expression, affinity purification is used to isolate and purify the recombinant Human UCHL5 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human UCHL5 protein. Its purity exceeds 90%.
Recombination of a plasmid encoding the Human UCHL5 protein (1-326aa) is the first step during the production the recombinant Human UCHL5 protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal 6xHis-SUMO tag. After expression, affinity purification is used to isolate and purify the recombinant Human UCHL5 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human UCHL5 protein. Its purity exceeds 90%.
| Cat.No | ACP04459 | Target Name | UCHL5 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 1-326aa | Mol Weight | 53.4kDa |
| Protein Length | Full Length of Isoform 4 | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9Y5K5 |
|---|
Uniprot Id
Q9Y5K5
Target Species
Human
Target Name
UCHL5
Target Full Name
Ubiquitin carboxyl-terminal hydrolase isozyme L5
Target Function
Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.
Target Subcellular Location
Cytoplasm. Nucleus. Note=Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus.
Target Protein Families
Peptidase C12 family
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
UCH37; AD-019; CGI 70; INO80 complex subunit R; INO80R; Ubiquitin C terminal hydrolase L5; Ubiquitin C terminal hydrolase UCH37; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin carboxyl terminal esterase L5; Ubiquitin carboxyl terminal hydrolase isozyme L5; Ubiquitin carboxyl terminal hydrolase L5; Ubiquitin carboxyl-terminal hydrolase isozyme L5; Ubiquitin thioesterase L5; Ubiquitin thiolesterase L5; UCH L5; UCH-L5; UCHL5; UCHL5_HUMAN
Target Background
Enables endopeptidase inhibitor activity; proteasome binding activity; and thiol-dependent deubiquitinase. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of smoothened signaling pathway; and protein deubiquitination. Located in cytosol; nucleolus; and nucleoplasm. Colocalizes with Ino80 complex.
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