Rabbit anti-Human BUB3 Monoclonal Antibody

The antibody against BUB3 was raised in Rabbit using a synthetic peptide corresponding to a sequence within amino acids 1-100 of human BUB3 (O43684) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, ELISA.

ADA-16120A

Number

Cat.No	ADA-16120A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	BUB3
Target Synonyms	BUB3L; hBUB3; BUB3	Form	Liquid
Species Reactivity	Human, Mouse, Rat	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.02% sodium azide, pH7.3.	Purification Method	Affinity purification
Positive Samples	HeLa, 293T, C2C12, MCF7, Mouse liver, Rat lung	Application	ELISA, WB, IF/ICC, IHC-P

Cat.No

ADA-16120A

Clonality

Monoclonal

Host Species

Rabbit

Target Name

BUB3

Target Synonyms

BUB3L; hBUB3; BUB3

Form

Liquid

Species Reactivity

Human, Mouse, Rat

Isotype

IgG

Storage Buffer

50% Glycerol, 0.05% BSA, PBS with 0.02% sodium azide, pH7.3.

Purification Method

Affinity purification

Positive Samples

HeLa, 293T, C2C12, MCF7, Mouse liver, Rat lung

Application

ELISA, WB, IF/ICC, IHC-P

Immunogen Description	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BUB3 (O43684).	Target Species	Human
Immunogen Sequence	MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIR	Uniprot ID	O43684

Immunogen Description

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BUB3 (O43684).

Target Species

Human

Immunogen Sequence

MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIR

Uniprot ID

O43684

Background Information

Uniprot Id

O43684
Target Species

Human
Target Name

BUB3
Target Full Name

Mitotic checkpoint protein BUB3
Target Function

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Target Subcellular Location

Nucleus. Chromosome, centromere, kinetochore.
Target Protein Families

WD repeat BUB3 family
Target Research Area

Cell Biology
Target Synonyms

Aa2-050; AU019800; AU021329; AU043350; AW146323; BUB 3; BUB 3L; BUB3 (budding uninhibited by benzimidazoles 3; yeast) homolog; bub3; BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3 mitotic checkpoint protein; BUB3_HUMAN; BUB3L; Budding uninhibited by benomyl; Budding uninhibited by benzimidazoles 3; Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae); Budding uninhibited by benzimidazoles 3 homolog (yeast); Budding uninhibited by benzimidazoles 3 homolog; Budding uninhibited by benzimidazoles 3; S. cerevisiae; homolog of; C78067; hBUB3; Mitotic checkpoint component; Mitotic checkpoint protein bub3; OTTHUMP00000020679; OTTHUMP00000020680; WD repeat type I transmembrane protein A72.5
Target Background

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Rabbit anti-Human BUB3 Monoclonal Antibody

Specifications

Immunogen Information

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