Rabbit anti-Human Phospho-p38 MAPK-T180 Monoclonal Antibody

The antibody against Phospho-p38 MAPK-T180 was raised in Rabbit using a synthetic phosphorylated peptide around T180 of human p38 MAPK (NP_620581.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.

ADA-13598A

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Western blot analysis of various lysates, using Phospho-p38 MAPK-T180 Rabbit mAb at 1:1000 dilution.293T and NIH3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Specifications

Cat.No	ADA-13598A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	Phospho-p38 MAPK-T180
Target Synonyms	MAPK14; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; p38; p38ALPHA; p38 MAPK; Phospho-p38 MAPK-T180	Form	Liquid
Species Reactivity	Human, Mouse, Rat	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	NIH/3T3, 293T	Application	ELISA, WB, IHC-P

Immunogen Information

Immunogen Description	A synthetic phosphorylated peptide around T180 of human p38 MAPK (NP_620581.1).	Target Species	Human
Immunogen Sequence	DEMTGY	Uniprot ID	Q16539, P53778, Q15759

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