Rabbit anti-Mouse ICAM-1/CD54 Monoclonal Antibody

The antibody against ICAM-1/CD54 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 28-485 of mouse ICAM-1/CD54(NP_034623.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, ELISA.

ADA-15553A

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Western blot analysis of various lysates, using ICAM-1/CD54 Rabbit mAb at 1:6000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Negative control (NC):C2C12
Exposure time: 15s.

Specifications

Cat.No	ADA-15553A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	ICAM-1/CD54
Target Synonyms	CD54; Ly-47; Icam-1; MALA-2; ICAM-1/CD54	Form	Liquid
Species Reactivity	Mouse, Rat	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	Mouse spleen, Rat lung	Application	ELISA, WB, IF/ICC, IHC-P

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 28-485 of mouse ICAM-1/CD54(NP_034623.1).	Target Species	Mouse
Uniprot ID	P13597	Immunogen Sequence

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