Rabbit anti-Mouse IL-1 alpha Monoclonal Antibody

The antibody against IL-1 alpha was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 115-270 of mouse IL-1 alpha (NP_034684.2) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.

ADA-15015A

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Western blot analysis of recombinant Mouse IL-1 alpha Protein, using IL-1 alpha Rabbit mAb at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Specifications

Cat.No	ADA-15015A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	IL-1 alpha
Target Synonyms	Il-1a; IL-1 alpha	Form	Liquid
Species Reactivity	Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	Recombinant Mouse IL-1 alpha Protein	Application	ELISA, WB

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 115-270 of mouse IL-1 alpha (NP_034684.2).	Target Species	Mouse
Immunogen Sequence	SAPYTYQSDLRYKLMKLVRQKFVMNDSLNQTIYQDVDKHYLSTTWLNDLQQEVKFDMYAYSSGGDDSKYPVTLKISDSQLFVSAQGEDQPVLLKELPETPKLITGSETDLIFFWKSINSKNYFTSAAYPELFIATKEQSRVHLARGLPSMTDFQIS	Uniprot ID	P01582

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