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The recombinant antibody against DOT1L was produced using a synthesized peptide derived from human KMT4 / Dot1L as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB.
The recombinant antibody against DOT1L was produced using a synthesized peptide derived from human KMT4 / Dot1L as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB.
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Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate All lanes: DOT1L antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 165 kDa Observed band size: 165 kDa
Immunofluorescence staining of Hela cell with DOT1L Recombinant Monoclonal Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).2
| Cat.No | ADC-57084A | Clonality | Monoclonal |
|---|---|---|---|
| Target Name | DOT1L | Target Synonyms | DOT1L, H3 lysine-79 specific (EC 2.1.1.43) (DOT1-like protein) (Histone H3-K79 methyltransferase) (H3-K79-HMTase) (Lysine N-methyltransferase 4), Histone-lysine N-methyltransferase, KIAA1814 KMT4 |
| Form | Liquid | Species Reactivity | Human |
| Isotype | Rabbit IgG | Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline |
| Purification Method | Affinity-chromatography purified | Conjugate | Non-conjugated |
| Application | ELISA, WB | Storage | Upon receipt |
| Immunogen Description | A synthesized peptide derived from human KMT4 / Dot1L | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q8TEK3 |
Uniprot Id
Q8TEK3
Target Species
Human
Target Name
DOT1L
Target Full Name
Histone-lysine N-methyltransferase, H3 lysine-79 specific
Target Function
Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
Target Subcellular Location
Nucleus.
Target Protein Families
Class I-like SAM-binding methyltransferase superfamily, DOT1 family
Target Synonyms
Disrupter of telomere silencing protein 1; DOT 1; DOT1; DOT1 like; DOT1 like histone H3 methyltransferase (S. cerevisiae); DOT1 like histone H3 methyltransferase; DOT1 like histone H3K79 methyltransferase; DOT1 like protein ; DOT1; S. cerevisiae; homolog of; DOT1-like protein; DOT1L; DOT1L_HUMAN; EC 2.1.1.43; H3 K79 HMTase; H3 lysine-79 specific; H3-K79-HMTase; Histone H3 K79 methyltransferase; Histone H3 methyltransferase; Histone H3 methyltransferase DOT1-like; Histone H3-K79 methyltransferase; Histone lysine N methyltransferase H3 lysine 79 specific; Histone methyltransferase DOT1L; Histone-lysine N-methyltransferase; KIAA1814; KMT4; Lysine N methyltransferase 4; Lysine N-methyltransferase 4
Target Background
The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes.
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