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The antibody against CD8A was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 28-196 of mouse CD8A(NP_001074579.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, FC, ELISA.
The antibody against CD8A was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 28-196 of mouse CD8A(NP_001074579.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, FC, ELISA.
Western blot analysis of various lysates, using CD8A Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
| Cat.No | ADA-15428A | Clonality | Monoclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | CD8A |
| Target Synonyms | Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; CD8A | Form | Liquid |
| Species Reactivity | Mouse | Isotype | IgG |
| Storage Buffer | 50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Mouse thymus | Application | ELISA, WB, FC |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 28-196 of mouse CD8A(NP_001074579.1) | Target Species | Mouse |
|---|---|---|---|
| Immunogen Sequence | KPQAPELRIFPKKMDAELGQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSAMRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSPVHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIY | Uniprot ID | P01731 |
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