Rabbit anti-Mouse IFN-beta Monoclonal Antibody

The antibody against IFN-beta was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of mouse IFN-beta (NP_034640.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.

ADA-16701A

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Western blot analysis of recombinant Mouse IFN-beta Protein using IFN-beta Rabbit mAb at 1:6000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 10ng/5ng/1ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Specifications

Cat.No	ADA-16701A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	IFN-beta
Target Synonyms	Ifb; IFNB; If1da1; IFN-beta	Form	Liquid
Species Reactivity	Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	Recombinant Mouse IFN-beta Protein	Application	ELISA, WB

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of mouse IFN-beta (NP_034640.1)	Target Species	Mouse
Immunogen Sequence	MNNRWILHAAFLLCFSTTALSINYKQLQLQERTNIRKCQELLEQLNGKINLTYRADFKIPMEMTEKMQKSYTAFAIQEMLQNVFLVFRNNFSSTGWNETIVVRLLDELHQQTVFLKTVLEEKQEERLTWEMSSTALHLKSYYWRVQRYLKLMKYNSYAWMVVRAEIFRNFLIIRRLTRNFQN	Uniprot ID	P01575

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