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The antibody against V5-Tag was raised in mouse using the GKPIPNPLLGLDST synthetic peptide conjugate to KLH as the immunogen. This antibody exists as a non-conjugated isotype IgG1, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IF, IP, FC.
The antibody against V5-Tag was raised in mouse using the GKPIPNPLLGLDST synthetic peptide conjugate to KLH as the immunogen. This antibody exists as a non-conjugated isotype IgG1, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IF, IP, FC.
$200.00
Western Blot Positive WB detected in: 50ng V5-tagged fusion protein V5 Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 37.8 KDa Observed band size: 43 KDa Exposure time: 10S
Western Blot Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:5000 Lane 1: Recombinant V5-tagged fusion protein at 50ng Lane 2: Recombinant V5-tagged fusion protein at 25ng Lane 3: Recombinant V5-tagged fusion protein at 12.25ng Lane 4: Recombinant V5-tagged fusion protein at 6.25ng Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 37.8 kd Observed band size: 43 kd Exposure time: 5min2
Western Blot Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4 at 50ng All lanes: V5 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 50.2, 47.6, 66.7, 37.8 KDa Observed band size: 52, 47, 67, 42 KDa Exposure time: 30s3
| Cat.No | ADC-57238A | Clonality | Monoclonal |
|---|---|---|---|
| Host Species | Mouse | Target Name | V5-Tag |
| Form | Liquid | Species Reactivity | ALL |
| Isotype | IgG1 | Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 |
| Purification Method | >95%, Protein G purified | Conjugate | Non-conjugated |
| Application | ELISA, IF, WB, FC, IP | Storage | Upon receipt |
| Immunogen Description | GKPIPNPLLGLDST synthetic peptide conjugate to KLH | Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
|---|
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