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The antibody against MIPEP was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 511-713 of human MIPEP (NP_005923.3) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
The antibody against MIPEP was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 511-713 of human MIPEP (NP_005923.3) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
Western blot analysis of various lysates, using MIPEP Rabbit mAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
| Cat.No | ADA-15521A | Clonality | Monoclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | MIPEP |
| Target Synonyms | MIP; HMIP; COXPD31; MIPEP | Form | Liquid |
| Species Reactivity | Human | Isotype | IgG |
| Storage Buffer | 50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | T-47D, U266 | Application | ELISA, WB, IF/ICC |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 511-713 of human MIPEP (NP_005923.3). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q99797 | Immunogen Sequence |
Uniprot Id
Q99797
Target Species
Human
Target Name
MIPEP
Target Full Name
Mitochondrial intermediate peptidase
Target Function
Cleaves proteins, imported into the mitochondrion, to their mature size.
Target Involvement
Combined oxidative phosphorylation deficiency 31 (COXPD31)
Target Subcellular Location
Mitochondrion matrix.
Target Protein Families
Peptidase M3 family
Target Synonyms
EC 3.4.24.59 ; HMIP; MIP; Mipep; MIPEP_HUMAN; Mitochondrial intermediate peptidase; Mitochondrial intermedieate peptide; OTTHUMP00000018121; OTTHUMP00000042292
Target Background
The product of this gene performs the final step in processing a specific class of nuclear-encoded proteins targeted to the mitochondrial matrix or inner membrane. This protein is primarily involved in the maturation of oxidative phosphorylation (OXPHOS)-related proteins. This gene may contribute to the functional effects of frataxin deficiency and the clinical manifestations of Friedreich ataxia.
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