Rabbit anti-Mouse IL-1RA Monoclonal Antibody

The antibody against IL-1RA was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 27-178 of mouse IL-1RA ( NP_001034790.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.

ADA-15462A

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Western blot analysis of various lysates, using IL-1RA Rabbit mAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

Specifications

Cat.No	ADA-15462A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	IL-1RA
Target Synonyms	IL-1ra; F630041P17Rik; IL-1RA	Form	Liquid
Species Reactivity	Human, Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	A-431	Application	ELISA, WB

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 27-178 of mouse IL-1RA ( NP_001034790.1).	Target Species	Mouse
Immunogen Sequence	PSGKRPCKMQAFRIWDTNQKTFYLRNNQLIAGYLQGPNIKLEEKIDMVPIDLHSVFLGIHGGKLCLSCAKSGDDIKLQLEEVNITDLSKNKEEDKRFTFIRSEKGPTTSFESAACPGWFLCTTLEADRPVSLTNTPEEPLIVTKFYFQEDQ	Uniprot ID	P25085

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