Rabbit anti-Human Fibrillarin/U3 RNP Polyclonal Antibody

Uniprot Id

P22087

Target Species

Human

Target Name

FBL

Target Full Name

rRNA 2'-O-methyltransferase fibrillarin

Target Function

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes. U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus.

Target Subcellular Location

Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Fibrillar region of the nucleolus.

Target Protein Families

Methyltransferase superfamily, Fibrillarin family

Target Synonyms

FIB; FLRN; Nop1; RNU3IP1; Fibrillarin/U3 RNP

Target Background

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.